1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、ca、sci等外文数据库检索资源上查阅有关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解解脂耶氏酵母胞质中乙酰-coa含量对菌体产油脂以及产间苯三酚的影响，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1]#160;#160;#160;#160; Yang F, Cao Y. Biosynthesis of phloroglucinol compounds in microorganisms[J]. Applied microbiology and biotechnology, 2012, 93(2): 487-495. [2]#160;#160;#160;#160; Achkar J, Xian M, Zhao H, et al. Biosynthesis of phloroglucinol[J]. Journal of the American Chemical Society, 2005, 127(15): 5332-5333. [3]#160;#160;#160;#160; Zha W, Rubin-Pitel S B, Shao Z, et al. Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering[J]. Metabolic engineering, 2009, 11(3): 192-198. [4]#160;#160;#160;#160; Zha W, Rubin-Pitel S B, Zhao H. Characterization of the substrate specificity of PhlD, a type III polyketide synthase from Pseudomonas fluorescens[J]. Journal of Biological Chemistry, 2006, 281(42): 32036-32047. [5]#160;#160;#160;#160; Cao Y, Jiang X, Zhang R, et al. Improved phloroglucinol production by metabolically engineered Escherichia coli[J]. Applied microbiology and biotechnology, 2011, 91(6): 1545-1552. [6]#160;#160;#160;#160; Zha W, Rubin-Pitel S B, Zhao H. Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity[J]. Molecular BioSystems, 2008, 4(3): 246-248. [7]#160;#160;#160;#160; Rao G, Lee J K, Zhao H. Directed evolution of phloroglucinol synthase PhlD with increased stability for phloroglucinol production[J]. Applied microbiology and biotechnology, 2013, 97(13): 5861-5867. [8]#160;#160;#160;#160; Abghari A, Chen S. Yarrowia lipolytica as an oleaginous cell factory platform for production of fatty acid-based biofuel and bioproducts[J]. Frontiers in Energy Research, 2014, 2: 21. [9]#160;#160;#160;#160; 孙美莉, 刘虎虎, 邬文嘉, 等. 代谢工程改造酵母生产多不饱和脂肪酸的研究进展[J]. 化工进展, 2016, 35(3): 872-878. [10] #160;Liu H H, Ji X J, Huang H. Biotechnological applications of Yarrowia lipolytica: past, present and future[J]. Biotechnology advances, 2015, 33(8): 1522-1546. [11] #160;Wasylenko T M, Ahn W S, Stephanopoulos G. The oxidative pentose phosphate pathway is the primary source of NADPH for lipid overproduction from glucose in Yarrowia lipolytica[J]. Metabolic engineering, 2015, 30: 27-39. [12] #160;Nicaud J M, Le Clainche A, Le Dall M T, et al. Yarrowia lipolytica, a yeast mo

3. 毕业设计（论文）进程安排

1.10-3.1 确定毕业设计的课题及相关资料的查找 3.2-3.10 实验方案的设计 3.10-3.20 实验材料的准备 3.21-5.15 准备分子实验 5.15-6.5 实验数据处理与结果分析 5.20-6.10 毕业论文书写