1. 毕业设计（论文）的内容和要求

手性胺和非天然的氨基酸在药物市场具有很大前景，现阶段接近40%的药物都是含有氨基的药物，如α-四氢萘胺（抗抑郁药舍曲林的关键结构），西他列汀（糖尿病治疗药物）等。

可利用ω-转氨酶生物催化方法对底物进行氨化，可以有效地的避免传统化学法的高压高温高能耗高污染，生产手性胺。

本课题将利用底物谱对ω-转氨酶进行表征，探寻不同氨基底物和氨基受体对于ω-转氨酶催化能力。

2. 参考文献

[1]贾红华, 朱清禾, 李艳, et al. ω-转氨酶制备手性胺的研究进展[J]. 现代化工, 2012, 32(3):16-20. [2]Koszelewski D , Tauber K , Faber K , et al. ω-Transaminases for the synthesis of non-racemic α-chiral primary amines[J]. Trends in Biotechnology, 2010, 28(6):324-332. [3]Koszelewski D , Clay D , Rozzell D , et al. Deracemisation of alpha-Chiral Primary Amines by a One-Pot, Two-Step Cascade Reaction Catalysed by omega-Transaminases[J]. European Journal of Organic Chemistry, 2009, 2009(14):4. [4]Koszelewski D , Iv#195;#161;n Lavandera, Clay D , et al. Asymmetric Synthesis of Optically Pure Pharmacologically Relevant Amines Employing -Transaminases[J]. Advanced Synthesis Catalysis, 2010, 350(17):2761-2766. [5]Kelly S A , Pohle S , Wharry S , et al. Application of ω-Transaminases in the Pharmaceutical Industry[J]. Chemical Reviews, 2017:acs.chemrev.7b00437. [6]Mathew S , Nadarajan S P , Sundaramoorthy U , et al. Biotransformation of β-keto nitriles to chiral (S)-β-amino acids using nitrilase and ω-transaminase[J]. Biotechnol Lett, 2017, 39(4):535-543. [7]Mahesh D P , Gideon G , Andreas B , et al. Recent Advances in ω-Transaminase-Mediated Biocatalysis for the Enantioselective Synthesis of Chiral Amines[J]. Catalysts, 2018, 8(7):254-. [8]Yun H , Hwang B Y , Lee J H , et al. Use of enrichment culture for directed evolution of the Vibrio fluvialis JS17 omega-transaminase, which is resistant to product inhibition by aliphatic ketones.[J]. Applied Environmental Microbiology, 2005, 71(8):4220-4. [9]Hwang B Y , Kim B G . High-throughput screening method for the identification of active and enantioselective ω-transaminases[J]. Enzyme Microbial Technology, 2004, 34(5):429-436. [10]Mutti F G , Fuchs C S , Pressnitz D , et al. Amination of Ketones by Employing Two New (S)-Selective ω-Transaminases and the His-Tagged ω-TA from Vibrio fluvialis[J]. European Journal of Organic Chemistry, 2012, 2012(5):1003-1007. [11]Seo Y M , Mathew S , Bea H S , et al. ω-Transaminases for the Production of Optically Pure Amines and Unnatural Amino Acids[J]. Organic Biomolecular Chemistry, 2012, 10(12):2482-2485. [12]Pressnitz D , Fuchs C S , Sattler J H , et al. Asymmetric Amination of Tetralone and Chromanone Derivatives Employing ω-Transaminases[J]. Acs Catalysis, 2016, 3(4):555#8211;559.

3. 毕业设计（论文）进程安排

2018.12.25~2018.12.31 下达课题任务，查阅文献，做好开题前期准备工作； 2018.12.31~2019.1.23 完善课题研究方案，完成外文翻译、文献综述和开题报告工作，组织开题论证和初期检查工作； 2019.1.23~2019.6.7 课题的实验、设计、调研及结果的处理与分析，完成毕业论文和答辩PPT的写作； 2019.6.7~2019.6.14 课题论文答辩。

2019.6.14~2019.7.5 完成毕业设计（论文）教学质量的分析、总结和评优工作，做好材料的收集、整理和归档工作。