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毕业论文网 > 任务书 > 化学化工与生命科学类 > 制药工程 > 正文

IPTG诱导蛋白原理及工艺表达任务书

 2020-05-24 12:16:45  

1. 毕业设计(论文)的内容和要求

内容:异丙基硫代半乳糖苷(iptg)是一种作用极强的诱导剂,不被细菌代谢而十分稳定,因此被实验室广泛应用。

实验主要探究其表达原理以及各种影响因素,并将其放大到工业生产中所遇到的问题及解决方法。

要求:掌握一定的文献查阅能力,合理设计实验,认真严格完成实验操作。

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2. 参考文献

[1] McBride AJ, Athanazio DA, Reis MG, Ko AI: Leptospirosis. Curr Opin Infect Dis 2005, 18(5):376#8211;386. [2] Ko AI, Goarant C, Picardeau M: Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat Rev Microbiol 2009, 7(10):736#8211;747. [3] F#233;lix SR, Hartwig DD, Argondizzo AP, Silva EF, Seixas FK, Neto AC, Medeiros MA, Lilenbaum W, Dellagostin OA: Subunit approach to evaluation of the immune protective potential of leptospiral antigens. Clin Vaccine Immunol 2011, 18(12):2026#8211;2030. [4] Nabity SA, Ribeiro GS, Aquino CL, Takahashi D, Dami#227;o AO, Gon#231;alves AH, Miranda-Filho DB, Greenwald R, Esfandiari J, Lyashchenko KP, Reis MG, Medeiros MA, Ko AI: Accuracy of a dual path platform (DPP) assay for the rapid point-of-care diagnosis of human leptospirosis. PLoS Negl Trop Dis 2012, 6(11):e1878. [5] Lin Y-P, Lee D-W, McDonough SP, Nicholson LK, Sharma Y, Chang Y-F: Repeated domains of Leptospira immunoglobulin-like proteins interact with elastin and tropoelastin. J Biol Chem 2009, 284(29):19380#8211;19391. [6] Lin YP, McDonough SP, Sharma Y, Chang YF: The terminal immunoglobulin-like repeats of LigA and LigB of Leptospira enhance their binding to gelatin binding domain of fibronectin and host cells. PLoS One 2010, 5(6):e11301. [7] Choy HA: Multiple activities of LigB potentiate virulence of Leptospira interrogans: inhibition of alternative and classical pathways of complement. PLoS One 2012, 7(7):e41566. [8] Volont#232; F, Marinelli F, Gastaldo L, Sacchi S, Pilone MS, Pollegioni L, Molla G: Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli. Protein Expr Purif 2008, 61 (2):131 #8211;137. [9] Pan H, Xie Z, Bao W, Zhang J: Optimization of culture conditions to enhance cis-epoxysuccinate hydrolase production in Escherichia coli by response surface methodology. Biochem Eng J 2008, 42(2):133#8211;138. [10] Papaneophytou CP, Kontopidis G: Statistical approaches to maximize recombinant protein expression in Escherichia coli: A general review. Protein Expr Purif 2014, 94:22#8211;32.

3. 毕业设计(论文)进程安排

2016.03.01-2016.03.10 查阅文献,了解实验内容,制定实验路线,购买所需的药品和仪器。

2016.03.11-2016.05.10 探究异丙基硫代半乳糖苷(iptg)表达原理以及各种影响因素,并研究工业化生产工艺。

2016.05.11-2016.05.31 撰写实验总结和毕业论文。

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