1. 毕业设计（论文）的内容和要求

黑水虻繁殖力强、营养物质含量丰富，关键是养殖成本极低，而产量却很高，还在一定程度上改善了人类赖以生存的环境。

这说明黑水虻适合大规模工业化养殖，黑水虻幼虫可为昆虫蛋白质的工业化生产提供充足的原料，并能大幅度降低天然蛋白质的生产成本。

国内外有关微生物、植物和哺乳动物蛋白质的提取分离及功能特性与理化特性的研究方法都已经很成熟，研究结果数不胜数。

2. 参考文献

[1] 许彦腾. 黑水虻幼虫蛋白质提取与抗氧化活性及理化特性研究[D]. 西北农林科技大学, 2015. [2] 杨晶，张敏娟，薛忠民，等. 桑树叶蛋白提取及蛋白粉制备工艺[J]. 蚕业科学. 2015(03): 518-524. [3] 刘冉. 六种常见养殖昆虫甲壳素提取的比较[D]. 河北大学, 2018. [4] 黄超，秦磊磊，沈侨，等. 黑水虻幼虫蛋白质分级提取及理化性质研究[J]. 化学与生物工程. 2019, 36(01): 26-30. [5] 徐齐云，喻国辉，安新城. 黑水虻蛹壳中几丁质的提取及壳聚糖制备研究[J]. 广东农业科学. 2012(05): 87-88. [6] Buszlig;ler S, Rumpold B A, Jander E, et al. Recovery and techno-functionality of flours and proteins from two edible insect species: Meal worm (Tenebrio molitor) and black soldier fly ( Hermetia illucens ) larvae[J]. Heliyon. 2016, 2(12): 218. [7] Tourtois J, Ali J G, Grieshop M J. Susceptibility of wounded and intact black soldier fly Hermetia illucens (L.) (Diptera: Stratiomyidae) to entomopathogenic nematodes[J]. Journal of Invertebrate Pathology. 2017, 150: 121-129. [8] Cutrignelli M I, Messina M, Tulli F, et al. Evaluation of an insect meal of the Black Soldier Fly (Hermetia illucens) as soybean substitute: Intestinal morphometry, enzymatic and microbial activity in laying hens[J]. Research in Veterinary Science. 2018, 117: 209-215. [9] Bulak P, Polakowski C, Nowak K, et al. Hermetia illucens as a new and promising species for use in entomoremediation[J]. Science of The Total Environment. 2018, 633: 912-919. [10] Lee Y, Seo S, Yoon S, et al. Identification of a novel alkaline amylopullulanase from a gut metagenome of Hermetia illucens[J]. International Journal of Biological Macromolecules. 2016, 82: 514-521. [11] Wasko A, Bulak P, Polak-Berecka M, et al. The first report of the physicochemical structure of chitin isolated from Hermetia illucens[J]. Int J Biol Macromol. 2016, 92: 316-320. [12] Lee C, Lee Y, Kim S, et al. Isolation and characterization of a halotolerant and protease-resistant α-galactosidase from the gut metagenome of Hermetia illucens[J]. Journal of Biotechnology. 2018, 279: 47-54. [13] Mart#237;nez-S#225;nchez A, Magantilde;a C, Salontilde;a M, et al. First record of Hermetia illucens (Diptera: Stratiomyidae) on human corpses in Iberian Peninsula[J]. Forensic Science International. 2011, 206(1-3): e76-e78. [14] Harnden L M, Tomberlin J K. Effects of temperature and diet on black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), development[J]. Forensic Science International. 2016, 266: 109-116. [15] Salomone R, Saija G, Mondello G, et al. Environmental impact of food waste bioconversion by insects: Application of Life Cycle Assessment to process using Hermetia illucens[J]. Journal of Cleaner Production. 2017, 140: 890-905. [16] Lalander C, Diener S, Zurbr#252;gg C, et al. Effects of feedstock on larval development and process efficiency in waste treatment with black soldier fly (Hermetia illucens)[J]. Journal of Cleaner Production. 2019, 208: 211-219. [17] Dobermann D, Field L M, Michaelson L V. Using Hermetia illucens to process Ugandan waragi waste[J]. Journal of Cleaner Production. 2019, 211: 303-308.

3. 毕业设计（论文）进程安排

2018.11.21 确定论文题目 2018.11.21#8212;2019.1.15 查找资料、完成开题报告 2019.2.20#8212;2019.3.10 准备实验 2019.3.10#8212;2019.5.20 完成实验 2019.5.21#8212;2019.5.30 完善实验、完成初稿 2019.6.1#8212;2019.6.9 论文修改 2019.6.12#8212;2019.6.16 论文答辩