1. 毕业设计（论文）的内容和要求

1．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2．开展实验研究 掌握厌氧发酵基本操作，设计实验方案，并按计划进行实验。

3．处理实验数据和产品检测 实验结束后，进行科学的记录和数据处理，对实验结果进行分析，归纳总结，提出改进方案和可行建议，得出相应的结论。

2. 参考文献

[1] Morris BEL, Henneberger R, Huber H, Eichinger CM. Microbial syntrophy: interaction for the common good [J]. FEMS Microbiol Rev, 2013, 37: 384-406. [2] Mcinerney MJ, Sieber JR, Gunsalus RP. Microbial syntrophy ecosystem level biochemical cooperation - genomic sequences reveal systems required to produce hydrogen and formate, plus other hallmarks of the syntrophic lifestyle [J]. Microbe, 2011, 6 (11): 479 -485. [3] 张小元, 李香真, 李家宝. 微生物互营产甲烷研究进展 [J]. 应用与环境生物学报, 2016, 22(1): 156-166. [4] Heidelberg JF, Paulsen IT, Nelson KE, et al. Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis[J]. Nature Biotechnology, 2002, 20(11): 1118-1123. [5] Xiao X, Wu YM, Xu CC, et al. Anaerobic respiratory capabilities of a metal-reducing microorganism Shewanella and its application in environmental remediation[J]. Microbiology China, 2012, 39(11): 1677-1686 (in Chinese). [6] Zou CH, Xu FC, Chen XH. Decolorization of dyes by a current-producing bacterium Shewanella marisflavi EP1 isolated from sea sediments[J]. Microbiology China, 2011, 38(1): 2-7 (in Chinese). [7] 刘鹏程, 朱雯雯, 肖翔. 产电微生物Shewanella菌厌氧呼吸代谢网络研究进展 [J]. 微生物学通报, 2015, 42(11): 2238-2244. [8] Rotaru A, Shrestha P, Liu F, et al. Interspecies electron transfer via hydrogen and formate rather than direct electrical connections in cocultures of Pelobacter ca rbinolicus and Geobacter sulfurreducens[J]. Applied Environmental Microbiology, 2012, 78(21): 7645-7651. [9] 张杰, 陆雅海. 互营氧化产甲烷微生物种间电子传递研究进展[J]. 微生物学通报, 2015, 42(5): 920-927. [10] 康嘉兴. 希瓦氏菌MR-1在共培养过程中胞外电子传递通路的研究[D]. 南京: 东南大学, 2016. [11] Miriam A Rosenbaum，Haim Y. Bar，Qasim K. Beg，Daniel Segre c, James Booth，Michael. Cotta, Largus. Shewanella oneidensis in a lactate-fed pure-culture and a glucose-fed co-culture with Lactococcus lactis with an electrode as electron acceptor [J]. Bioresource Technology, 2011, 102 : 2623-2628. [12] Liang Fu a, Shan-Wei Li a, Zhao-Wei Ding a, Jing Ding a, b, Yong-Ze Lu a, Raymond J. Zeng. Iron reduction in the DAMO/Shewanella oneidensis MR-1 coculture system and the fate of Fe(II) [J]. Water Research, 2016, 88: 808-815. [13] Shanshan Chen , Amelia-Elena Rotaru , Fanghua Liu , Jo Philips , Trevor L. Woodard , Kelly P. Nevin , Derek R. Lovley. Carbon cloth stimulates direct interspecies electron transfer in syntrophic co-cultures[J]. Bioresource Technology, 2014, 173:82-86 [14] Chen, S., Rotaru, A.-E., Shrestha, P.M., Malvankar, N.S., Liu, F., Fan, W., Nevin, K.P., Lovley, D.R., 2014. Promoting interspecies electron transfer with biochar. Sci. Rep. 4 (5019). http://dx.doi.org/10.1038/srep05019. [15] 甘荣,葛明民,刘勇迪,贾红华,闰志英,雍晓雨, 吴夏芫,周俊. 活性炭在中高温条件下对玉米秸秆厌氧发酵的影响[J]. 环境科学, 2017, 38(6): 2607-2616. [16] 马晨, 周顺桂, 庄莉等. 微生物胞外呼吸电子传递机制研究进展[J]. 生态学报, 2011, 31(7): 2008-2018. [17] Fanghua Liu, Amelia-Elena Rotaru, Pravin M. Shrestha, Nikhil S. Malvankar, Kelly P. Nevin. Promoting direct interspecies electron transfer with activated carbon[J]. Energy Environmental Science, Sci. 2012, 5,(8982 ). http://www.rsc.org/ees

3. 毕业设计（论文）进程安排

01.01-03.01 确定毕业设计的课题及相关资料的查找 03.02-03.10 实验方案的设计 03.10-03.20 实验材料的准备 03.21-05.15 完成实验并记录数据 05.15-06.05 实验数据处理与结果分析 05.20-06.10 毕业论文书写