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毕业论文网 > 任务书 > 化学化工与生命科学类 > 制药工程 > 正文

在藤仓赤霉菌中建立高效crispr/cas9基因组编辑技术研究任务书

 2020-06-25 20:49:04  

1. 毕业设计(论文)的内容和要求

1.文献查阅 掌握文献查阅的一般方法,学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、ca、sci等外文数据库检索资源上查阅有关文献。

2.文献阅读及综述 阅读与课题相关的外文及中文文献,了解藤仓赤霉菌分子与发酵方面的文献,撰写文献综述。

3.明确实验任务,拟定实验方案 根据所查阅文献的内容,确定实验内容及方案,拟定科学可行的研究计划。

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2. 参考文献

[1].Pohl C, Kiel J A, Driessen A J, et al. CRISPR/Cas9 Based Genome Editing of Penicillium chrysogenum.[J]. Acs Synthetic Biology, 2016, 5(7):754-764. [2].Arazoe T, Ogawa T, Miyoshi K, et al. Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus[J]. Biotechnology and Bioengineering, 2015, 112(7):1335#8211;1342. [3].Ninomiya Y, Suzuki K, Ishii C, et al. Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(33):12248-12253. [4].Zhang C, Meng X, Wei X, et al. Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus[J]. Fungal Genetics Biology, 2016, 86:47-57. [5].Schuster M, Schweizer G, Reissmann S, et al. Genome editing in Ustilago maydis, using the CRISPR#8211;Cas system[J]. Fungal Genetics Biology, 2016, 89:3-9. [6].Liu R, Chen L, Jiang Y, et al. Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system[J]. Cell Discovery, 2015, 1:15007. [7].Fang Y, Tyler B M. Efficient Disruption and Replacement of an Effector Gene in the Oomycete Phytophthora sojae using CRISPR/Cas9.[J]. Molecular Plant Pathology, 2016, 17(1):127#8211;139. [8].Fuller K K, Chen S, Loros J J, et al. Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus[J]. Eukaryotic Cell, 2015, 25(2):: 709#8211;712. [9].Katayama T, Tanaka Y, Okabe T, et al. Development of a genome editing technique using the CRISPR/Cas9 system in the industrial filamentous fungus Aspergillus oryzae[J]. Biotechnology Letters, 2016, 38(4):637-642. [10].Tudzynski B, Houml;lter K. Gibberellin Biosynthetic Pathway in Gibberella fujikuroi: Evidence for a Gene Cluster[J]. Fungal Genetics Biology, 1999, 25(3):157-170. [11].Tudzynski B, Hedden P, Carrera E, et al. The P450-4 gene of Gibberella fujikuroi encodes ent-kaurene oxidase in the gibberellin biosynthesis pathway.[J]. Applied Environmental Microbiology, 2001, 67(8):3514-3522. [12].Pia Linnemannstouml;ns, Jessica Schulte, Maria del Mar Prado, et al. The polyketide synthase gene pks4, from Gibberella fujikuroi, encodes a key enzyme in the biosynthesis of the red pigment bikaverin[J]. Fungal Genetics Biology, 2002, 37(2):134-148. [13].Teichert S, Wottawa M, Schouml;nig B, et al. Role of the Fusarium fujikuroi TOR kinase in nitrogen regulation and secondary metabolism.[J]. Eukaryotic Cell, 2006, 5(10):1807-19. [14].Troncoso C, C#225;rcamo J, Hedden P, et al. Influence of electron transport proteins on the reactions catalyzed by Fusarium fujikuroi, gibberellin monooxygenases[J]. Phytochemistry, 2008, 69(3):672-683. [15]. Albermann S, Linnemannstouml;ns P, Tudzynski B. Strategies for strain improvement in Fusarium fujikuroi: overexpression and localization of key enzymes of the isoprenoid pathway and their impact on gibberellin biosynthesis.[J]. Applied Microbiology and Biotechnology, 2013, 97(7):2979-2995.

3. 毕业设计(论文)进程安排

2.10-3.1 确定毕业设计的课题及相关资料的查找 3.2-3.10 实验方案的设计 3.10-3.20 实验材料的准备 3.21-5.15 准备分子实验 5.15-6.5 实验数据处理与结果分析 5.20-6.10 毕业论文书写

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