1. 毕业设计（论文）的内容和要求

1.毕业论文内容 （1）根据自己的课题查阅文献，写出文献综述，对实验进行小试，并设计合理的实验方案。

（2）改变微囊藻生长环境#8212;#8212;光照和温度，通过酶联免疫法，测定纳米氧化锌胁迫下，微囊藻藻毒素的释放和生理生化响应。

（3）根据单因素实验结果设计正交实验，探究最佳处理条件。

2. 参考文献

[1]Angel, B.M., Vallotton, P., Apte, S.C. 2015. On the mechanism of nanoparticulate CeO#61490; toxicity to freshwater algae. Aquat. Toxicol. 168, 90-97. [2]Aruoja, V., Dubourguier, H.-C., Kasemets, K., Kahru, A. 2009. Toxicity of nanoparticles of CuO, ZnO and TiO2 to microalgae Pseudokirchneriella subcapitata. Sci. Total. Environ. 407, 1461-1468. [3]Bhuvaneshwari, M., Iswarya, V., Archanaa, S., Madhu, G.M., Kumar, G.K., Nagarajan, R., Chandrasekaran, N., Mukherjee, A. 2015. Cytotoxicity of ZnO NPs towards fresh water algae Scenedesmus obliquus at low exposure concentrations in UV-C, visible and dark conditions. Aquat. Toxicol. 162, 29-38. [4]Bleiwas, D.I. 2013. Potential for recovery of cerium contained in automotive catalytic converters. Center for Integrated Data Analytics Wisconsin Science Center. [5]Bormans, M., Lengronne, M., Brient, L., Duval, C. 2014. Cylindrospermopsin accumulation and release by the benthic cyanobacterium Oscillatoria sp. PCC 6506 under different light conditions and growth phases. Bull. Environ. Contam. Toxicol. 92, 243-247. [6]Chen, L., Mao, F., Kirumba, G.C., Jiang, C., Manefield, M., He, Y. 2015. Changes in metabolites, antioxidant system, and gene expression in Microcystis aeruginosa under sodium chloride stress. Ecotoxicol. Environ. Saf. 122, 126-135. [7]Collin, B., Oostveen, E., Tsyusko, O.V., Unrine, J.M. 2014. Influence of natural organic matter and surface charge on the toxicity and bioaccumulation of functionalized ceria nanoparticles in Caenorhabditis elegans. Environ. Sci. Technol. 48, 1280-1289. [8]Dalai, S., Pakrashi, S., Bhuvaneshwari, M., Iswarya, V., Chandrasekaran, N., Mukherjee, A. 2014. Toxic effect of Cr(VI) in presence of n-TiO2 and n-Al2O3 particles towards freshwater microalgae. Aquat. Toxicol. 146, 28-37. [9]Donald. M, A. 2009. Approaches to monitoring, control and management of harmful algal blooms (HABs). Ocean. Coast. Manage. 52, 342-347. [10]Engstrouml;m-Ouml;st, J., Repka, S., Mikkonen, M. 2011. Interactions between plankton and cyanobacterium Anabaena with focus on salinity, growth and toxin production. Harmful. Algae. 10, 530-535. [11]Fang, X., Yu, R., Li, B., Somasundaran, P., Chandran, K. 2010. Stresses exerted by ZnO, CeO2 and anatase TiO2 nanoparticles on the Nitrosomonas europaea. J. Colloid Interface Sci. 348, 329-334. [12]Gao, Y., Gao, N., Deng, Y., Gu, J., Shen, Y., Wang, S. 2012. Adsorption of microcystin-LR from water with iron oxide nanoparticles. Water. Environ. Res. 84, 562-568. [13]Han, J., Lee, S., Choi, K., Kim, J., Ha, D., Lee, C.-G., An, B., Lee, S.-H., Mizuseki, H., Choi, J.-W., Kang, S. 2016. Effect of nitrogen doping on titanium carbonitride-derived adsorbents used for arsenic removal. J. Hazard. Mater. 302, 375-385. [14]Hartmann, N.B., Baun, A. 2010. The nano cocktail-cotoxicological effects of engineered nanoparticles in chemical mixtures. Integr. Environ. Assess. Manag. 6, 311-313. [15]Hou, J., Yang, Y., Wang, P., Wang, C., Miao, L., Wang, X., Lv, B., You, G., Liu, Z. 2016. Effects of CeO2, CuO, and ZnO nanoparticles on physiological features of Microcystis aeruginosa and the production and composition of extracellular polymeric substances. Environ. Sci. Pollut. Res. , 1-10. [16]Huang, J., Cheng, J., Yi, J. 2016. Impact of silver nanoparticles on marine diatom Skeletonema costatum. J. Appl. Toxicol. 36, 1343-1354.

3. 毕业设计（论文）进程安排

2017.12.23-2018.02.01 查阅相关文献，完善课题方案 2018.02.02-2018.02.28 完成外文翻译和开题报告 2018.03.01-2018.04.08 完成光照条件改变的实验 2018.04.09-2018.04.30 完成温度改变的实验 2018.05.01-2018.05.20 完成毕业论文的撰写 2018.05.21-2018.06.02 完成毕业论文的修改和完善