1. 毕业设计（论文）的内容和要求

分析若干指定菌株的基因组，以降解玉米赤霉烯酮为例，筛选其中氧化还原反应酶和（脂）水解酶，构建一个综合蛋白质序列、催化位点、结合位点与结合底物、3D结构、及（部分）功能注释的酶数据库。

2. 参考文献

1. Zhao S, Kumar R, Sakai A, Vetting MW, Wood BM, Brown S, Bonanno JB, Hillerich BS, Seidel RD, Babbitt PC et al: Discovery of new enzymes and metabolic pathways by using structure and genome context. Nature 2013, 502(7473):698-702. 2. Barriuso J, Martinez MJ: In silico metagenomes mining to discover novel esterases with industrial application by sequential search strategies. J Microbiol Biotechnol 2015, 25(5):732-737. 3. Chatonnet A, Marchot P, Bourne Y, Lenfant N, Hotelier T: ESTHER database: update on the alpha/beta hydrolase fold superfamily of proteins. J Neurochem 2017, 142:209-209. 4. Leone P, Comoletti D, Taylor P, Bourne Y, Marchot P: Structure-function relationships of the alpha/beta-hydrolase fold domain of neuroligin: A comparison with acetylcholinesterase. Chem-Biol Interact 2010, 187(1-3):49-55. 5. Davids T, Schmidt M, Bottcher D, Bornscheuer UT: Strategies for the discovery and engineering of enzymes for biocatalysis. Curr Opin Chem Biol 2013, 17(2):215-220. 6. Veitch NC: Horseradish peroxidase: a modern view of a classic enzyme. Phytochemistry 2004, 65(3):249-259. 7. Yamaguchi M, Sato S: Spectrometric assay for horseradish peroxidase activity based on the linkage of conjugated system formed by oxidative decarboxylation. Spectrochim Acta A Mol Biomol Spectrosc 2017, 174:189-194.

3. 毕业设计（论文）进程安排

2月下旬-3月中旬：文献检索、数据准备。

3月中旬--5月中旬：构建、修正数据库，应用举例。

5月中旬--六月上旬：论文写作、答辩。