1. 毕业设计（论文）的内容和要求

毕业论文包括文献综述，合成实验及表征，探针检测，总结，及参考文献等几个部分。

在文献综述中，需要综述近年针对半乳糖苷酶，基于比色及荧光底物的新探针，并阐述其探针基本机理，如ict，fret等。

在实验部分，需要完成探针化合物的合成，纯化及表征图谱解析等，需要学生学习过滤，萃取，干燥，柱层析等单元操作。

2. 参考文献

[1] Qingping Wang, Ute Dechert, Frank Jirik, S.G. Withers C. SUICIDE INACTIVATION OF HUMAN PROSTATIC ACID PHOSPHATASE AND A PHOSPHOTYROSINE PHOSPHATASE. 1994,200, 577-583. [2] P. Vasu Govardhana Reddy, C. Suresh Reddy, and M. Venugopal. Synthesis and Antimicrobial Activity of N-Substituted N-[6-Methyl-2-oxido-1,3,2- dioxaphosphinino(5,4-b)pyridine-2-yl]ureas. Heteroatom Chemistry. 2013, 14, 6. [3] H. F. Freeman, C. W. Colver. Synthesis of Disodium Phenyl Phosphate. [4] Chae Yeong Kim,#8224; Hyo Jin Kang,#8224; Sang J. Chung,*,#8224; Hyun-Kyung Kim,*,#8225; Sang-Yun Na,#167; and Hae-Jo Kim*. Mitochondria-Targeting Chromogenic and Fluorescence Turn-On Probe for the Selective Detection of Cysteine by Caged Oxazolidinoindocyanine. Anal. Chem. 2016, 88, 7178#8722;7182. [5] Tomohiro Doura, Mako Kamiya,* Fumiaki Obata, Yoshifumi Yamaguchi, Takeshi Y. Hiyama, Takashi Matsuda, Akiyoshi Fukamizu, Masaharu Noda, Masayuki Miura, and Yasuteru Urano*.Detection of LacZ-Positive Cells in Living Tissue with Single-Cell Resolution. Angew. Chem. Int. Ed. 2016, 55, 9620 #8211;9624. [6] Hiroki Ito , Yu Kawamata , Mako Kamiya,* Kayoko Tsuda-Sakurai, Shinji Tanaka, Tasuku Ueno, Toru Komatsu, Kenjiro Hanaoka, Shigeo Okabe, Masayuki Miura, and Yasuteru Urano*. Red-Shifted Fluorogenic Substrate for Detection of lacZ-Positive Cells in Living Tissue with Single-Cell Resolution. Angew. Chem. Int. Ed. 2018, 57, 15702 #8211;15706.

3. 毕业设计（论文）进程安排

~2019.2.25 外文翻译及课题熟悉。

2019.2.26~2019.4.30 论文综述及探针分子合成。

2019.5.1~2019.5.15 探针分子应用及半乳糖苷酶检测。