大肠杆菌理性代谢改造构建L-苹果酸高产菌株任务书
2020-05-22 21:10:45
1. 毕业设计(论文)的内容和要求
1.文献查阅
掌握文献查阅,使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源,查阅关于大肠杆菌理性代谢改造方面的相关文献。
2.文献阅读及综述
2. 参考文献
[1] ohno y,nakamori t,z heng h,et al. reverse reaction of malic enzyme for hco
3-fixation into pyruvic acid to synthesize l -malic acid with enzymatic coenzyme regeneration [j]. biosci biotechnol biochem,2008,72:1278-1282.
[2] zheng h,ohno y,nakamori t,et al. production of l-malic acid with fixation of hco3-by malic enzyme-catalyzed reaction based on regeneration of coenzyme on electrode modified by layer-by-layer self-assembly method[j]. j biosci bioeng,2009,107:16-20.
3. 毕业设计(论文)进程安排
2015.12-2016.2 |
文献查阅,完成开题报告,熟悉实验仪器。 |
2016.3-2016.4 |
构建大肠杆菌基因敲除表达框 |
2016.4-2016.5 |
敲除sfcA和maeB,最终获得目的菌株 |
2016.5-2016.6 |
重组菌株E.coli BA071-PCK、BA072-PCK和BA073-PCK血清瓶发酵验证。 |