1. 毕业设计（论文）的内容和要求

1 文献阅读与归纳，深入了解课题背景 2 构建过表达邻苯二酚1,2-双加氧酶的重组菌 3 全细胞催化体系构建 4 独立完成毕业论文的撰写

2. 参考文献

1 Han L, Liu P, Sun J, et al. Engineering catechol 1, 2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in Escherichia coli[J]. Scientific reports, 2015, 5: 13435. 2 Long Y, Yang S, Xie Z, et al. Cloning, expression, and characterization of catechol 1, 2-dioxygenase from a phenol-degrading Candida tropicalis JH8 strain[J]. Preparative Biochemistry and Biotechnology, 2016, 46(7): 673-678. 3 Guzik U, Hupert-Kocurek K, Sitnik M, et al. High activity catechol 1, 2-dioxygenase from Stenotrophomonas maltophilia strain KB2 as a useful tool in cis, cis-muconic acid production[J]. Antonie van Leeuwenhoek, 2013, 103(6): 1297-1307.

3. 毕业设计（论文）进程安排

2018.12-2019.01 阅读文献，完成开题报告 2019.03-2019.04 重组大肠杆菌构建与验证 2019.05-2019.05 全细胞催化合成粘康酸 2019.06-2019.06 撰写毕业论文