1. 毕业设计（论文）的内容和要求

1．文献查阅
掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于遗传转化体系构建的相关文献。

2．文献阅读及综述
阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案
根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Asahina E, Tanno K. A large amount of trehalose in a frost-resistant insect [J]. Nature, 1964, 204(4964): 1222-1222.
[2] Becker A, Schl D P, Steele J, et al. The regulation of trehalose metabolism in insects [J]. Experientia, 1996, 52(5): 433-439.
[3] Albertorio F, Chapa V A, Chen X, et al. The α, α-(1→1) linkage of trehalose is key to anhydrobiotic preservation [J]. Journal of the American Chemical Society, 2007, 129(34): 10567-10574.
[4] Leslie S B, Israeli E, Lighthart B, et al. Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying [J]. Applied and Environmental Microbiology, 1995, 61(10): 3592-3597.
[5] Donnamaria M C, Howard E I, Griger J R. Interaction of water with α, α-trehalose in solution: molecular dynamics simulation approach [J]. Journal of the Chemical Society, Faraday Transactions, 1994, 90(18): 2731-2735.
[6] Crowe J H, Caroenter J F, Crowe L M, et al. Are freezing and dehydration similar stress vectors? A comparison of modes of interaction of stabilizing solutes with biomolecules [J]. Cryobiology, 1990, 27(3): 219-231.
[7] Schiraldi C, Lemia D I, Mario D R, et al. Trehalose production: exploiting novel approaches [J]. Trends in Biotechnology, 2002, 20(10): 420~425.
[8] Jesse m McFarland, David rabuka. Recent Advances in chemoenzymatic bioconjugation methods [J]. Organic Chemistry Insights, 2005, 5: 7~14.
[9] Samuel C Reddington, Mark Howarth. Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher [J]. Current Opinion in Chemical Biology, 2015, 29: 94~99.
[10] 曲茂华, 张凤英, 何明芳, 等. 海藻糖生物合成及应用研究进展 [J]. 食品工业科技. 2014, 16(35): 358-362.
[11] 任媛嫒, 刘景芳, 戴秀玉, 等. 海藻糖代谢途径相关基因及生物工程 [J]. 微生物学报 . 2003, 1(43): 682l~6825.
[12] 吴秀丽. 海藻糖合酶的研究进展 [J]. 微生物学通报 . 2009, 36(7): 1067~1072.
[13] 谢雪原, 周华, 高震, 等. 多酶偶联反应体系的研究和应用 [J]. 化工进展. 2004, 8(23): 864~868.
[14] 冯莹莹, 刘均忠, 张宏娟, 等. 双酶偶联生物合成L-酪氨酸 [J]. 精细化工. 2014, 5(31): 570~574.
[15] 冷静, 郑禹, 曾霞, 等. MBP-CsgAⅢ融合蛋白的表达和纯化 [J]. 免疫学杂志. 2006, 4(22): 390-392.

3. 毕业设计（论文）进程安排