1. 毕业设计（论文）的内容和要求

1、 内容 在试验过程中，制定出一套试验方案，也就是具体步骤，即：1、细胞培养（换液与传代）以及mtt的测定2、细胞给药造模3、细胞侵袭与迁移的研究4、数据处理。

本研究以人骨肉瘤细胞mg-63为研究对象，以冬凌草甲素诱导其凋亡，对细胞侵袭与迁移的各项指标进行测定，并对测得数据进行处理。

2、要求 （1）掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，sci等检索资源上查阅关于细胞凋亡的相关文献。

2. 参考文献

1. Watson AJ, Collins PD. Colon cancer: a civilization disorder. Dig Dis. 2011;29(2):222#8211;8. 2. Christofori G. New signals from the invasive front. Nature. 2006;441(7092):444#8211;50. 3. Fidler IJ. The pathogenesis of cancer metastasis: the ”seed and soil#8217; hypothesis revisited. Nat Rev Cancer. 2003;3(6):453#8211;8. 4. Thiery JP. Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer. 2002;2(6):442#8211;54. 5. Thiery JP, Acloque H, Huang RY, Nieto MA. Epithelial-mesenchymal transitions in development and disease. Cell. 2009;139(5):871#8211;90. 6. Guarion M. Epithelial-mesenchymal transition and tumour invasion. Int J Biochem Cell Biol. 2007;39(12):2153#8211;60. 7. Wendt MK, Schiemann WP. Therapeutic targeting of the focal adhesion complex prevents oncogenic TGF-beta signaling and metastasis. Breast Cancer Res. 2009;11(5):R68. 8. Tian M, Neil JR, Schiemann WP. Transforming growth factor-beta and the hallmarks of cancer. Cell Signal. 2011;23(6):951#8211;62. 9. Tirino V, Camerlingo R, Bifulco K, Irollo E, Montella R, Paino F, et al. TGF-beta1 exposure induces epithelial to mesenchymal transition both in CSCs and non-CSCs of the A549 cell line, leading to an increase of migration ability in the CD133 A549 cell fraction. Cell Death Disease. 2013;4:e620. 10. Shankar S, Nall D, Tang SN, Meeker D, Passarini J, Sharma J, et al. Resveratrol inhibits pancreatic cancer stem cell characteristics in human and KrasG12D transgenic mice by inhibiting pluripotency maintaining factors and epithelial-mesenchymal transition. PLoS One. 2011;6(1):e16530. 11. Sun C, Hu Y, Liu X, Wu T, Wang Y, He W, et al. Resveratrol downregulates the constitutional activation of nuclear factor-B in multiple myeloma cells, leading to suppression of proliferation and invasion, arrest of cell cycle and induction of apoptosis. Cancer Genet Cytogenet. 2006;165(1):9#8211;19. 12. Vergara D, Simeone P, Toraldo D, Del Boccio P, Vergaro V, Leporatti S, et al. Resveratrol downregulates Akt/GSK and ERK signaling pathways in OVCAR-3 ovarian cancer cells. Mol Biosyst. 2012;8(4):1078#8211;87.

3. 毕业设计（论文）进程安排

2月 确定毕业设计的课题及相关资料的查找 3月 完成相关的材料准备；完成开题报告 实验方法的确定及实验条件的优化 4月 实验阶段 5月 各项指标作比较，实验数据处理 6月 毕业论文的书写 在导师指导下修改论文，完成答辩