1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于多糖分离纯化及表征的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Raessler M. Sample preparation and current applications of liquid chromatography for the determination of non-structural carbohydrates in plants. TrAC - Trends in Analytical Chemistry. 2011; 30: 1833-1843. [2] Tonova K. Separation of poly- and disaccharides by biphasic systems based on ionic liquids. Separation and Purification Technology. 2012; 89: 57-65. [3] Qureshi RN, Kok WT. Application of flow field-flow fractionation for the characterization of macromolecules of biological interest: A review. Analytical and Bioanalytical Chemistry. 2011; 399: 1401-1411. [4] Persin Z, Stana-Kleinschek K, Foster TJ, Van Dam JEG, Boeriu CG, Navard P. Challenges and opportunities in polysaccharides research and technology: The EPNOE views for the next decade in the areas of materials, food and health care. Carbohydrate Polymers. 2011; 84: 22-32. [5] Zhang Y, Dai L, Kong X, Chen L. Characterization and in vitro antioxidant activities of polysaccharides from Pleurotus ostreatus. International Journal of Biological Macromolecules. 2012; 51: 259-265. [6] Barclay T, Ginic-Markovic M, Johnston MR, Cooper PD, Petrovsky N. Analysis of the hydrolysis of inulin using real time 1H NMR spectroscopy. Carbohydrate Research. 2012; 352: 117-125. [7] Chi L, Munoz EM, Choi HS, Ha YW, Kim YS, Toida T, Linhardt RJ. Preparation and structural determination of large oligosaccharides derived from acharan sulfate. Carbohydr. Res. 2006; 341: 864-869. [8] Gesteira TF, Coulson-Thomas VJ, Ogata FT, Farias EHC, Cavalheiro RP, De Lima MA, Cunha GLA, Nakayasu ES, Almeida IC, Toma L, Nader HB. A novel approach for the characterisation of proteoglycans and biosynthetic enzymes in a snail model. Biochim. Biophys. Acta Proteins Proteomics. 2011; 1814: 1862-1869. [9] Kim BJ, No YA, Lee Y, Kim MN, Hong CK, Yoo KH, Kim YM, Hwang JH, Kong SY. Use of cream containing mucus secreted by snails has an anti-aging effect on skin. Korean J. Dermatol. 2015; 53: 430-436. [10] Kim HS, Lee YH, Lee YR, Im SA, Lee JK, Yeong SK, Sim JS, Hyung SC, Lee CK. Activation of professional antigen presenting cells by acharan sulfate isolated from giant african snail, Achatina fulica. Arch. Pharmacal Res. 2007; 30: 866-870. [11] Lee YS, Yang HO, Shin KH, Choi HS, Jung SH, Kim YM, Oh DK, Linhardt RJ, Kim YS. Suppression of tumor growth by a new glycosaminoglycan isolated from the African giant snail Achatina fulica. Eur. J. Pharmacol. 2003; 465: 191-198. [12] Pomin VH. NMR structural determination of unique invertebrate glycosaminoglycans endowed with medical properties. Carbohydr. Res. 2015; 413: 41-50. [13] Ten Dam GB, Van De Westerlo EMA, Smetsers TFCM, Willemse M, Van Muijen GNP, Merry CLR, Gallagher JT, Kim YS, Van Kuppevelt TH. Detection of 2-O-suifated iduronate and N-acetylglucosamine units in heparan sulfate by an antibody selected against acharan sulfate (IdoA2S-GlcNAc)n. J. Biol. Chem. 2004; 279: 38346-38352. [14] Wang H, Zhao J, Li D, Wen C, Liu H, Song S, Zhu B. Comparison of polysaccharides of Haliotis discus hannai and Volutharpa ampullacea perryi by PMP-HPLC-MSn analysis upon acid hydrolysis. Carbohydr. Res. 2015; 415: 48-53.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.1.4-2017.1.13 文献查阅，研究方案制定和开题报告写作 2017.1.14-2017.1.24 开题报告的修改与复核 2017.2.4-2017.2.20 实践内容的准备 2017.2.21-2017.5.10 实践 2017.5.11-2017.5.15 实践内容处理与结果分析 2017.5.16-2017.6.1 毕业论文书写