催化核苷类药物合成的核糖磷酸化酶的克隆表达任务书
2020-05-26 20:39:17
1. 毕业设计（论文）的内容和要求
1．文献查阅
掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源，查阅关于核糖磷酸化酶产生菌产酶条件优化方面的相关文献。
2．文献阅读及综述
2. 参考文献
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[1] 黄炯威,莫世艺,刘宁等.基因工程菌发酵表达核苷磷酸化酶条件的优化[J]. China Pharmaceuticals,2014, 23(7): 22-24 [2] 韩明明,李爱星, 张京玉等.嘌呤衍生物的合成及初步抗肿瘤活性评价[J].科学技术与工程,2013,13(17):4773-6 [3] 闫蓬勃,邱蔚然,丁庆豹.核苷磷酸化酶的产酶条件优化的研究[J].药物生物技术,2001,8(2):83-86. [4] 邱蔚然,丁庆豹.酶法合成核苷类抗病毒药物[J].中国医药工业杂志:1999, 30(10)474-478 [5]Bzowska A,Kulikowska E,Shugar D.Purine nucleoside phosphorylases:Proper-ties,functions and clinical aspects[J].Pharmacologyamp;Therapentics,2000,88:349-425. [6]Walter M R,Cook W J.Three-dimensional structure of thymidine phosphorylase fromEscherichia coliat 2.8A resolution [J].The Journal of Biological Chemistry,1999,23:14016-14022. [7]Esipov R S,Gurevich A I.Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/BL21 (DE3)system yields active recombinant enzymes[J].Protein Expression and Purification,2002,24:56-60. [8] Utagawa T,Morisawa H,Yamanaka S,et al.Mechanism of purine arabinoside synthesis by bacterial transarabinosylation reaction[J].Agric Biol Chem,1985,49(8):2425-2430. [9] Shirae H,Yokozeki K,Kubota K.Enzymatic production of ribavirin from pyrimidine nucleosides by Enterobacter aerogenesAJ 11125[J].Agric Biol Chem,1988,52(5):1233-1237. [10]Shirae H,Yokozeki,Kubota K.Enzymatic production of ribavirin from orotidine by Erwinia carotovoraAJ 2992[J].Agric Biol Chem,1988,52(6):1499-1504. [11] Kroon J, Souer E, Graaff A, et al.Cloning and structural analysis of the anthocyanin pigmentation locus Rt of Petunia hybrida: characterization of insertion sequences in two mutant alleles. The Plant Journal,1994, 5(1):69~80.8 [12]Pugmire MJ ,Ealick SE.Strutural analyses reveal two distinct families of nucleoside phosphoiylases .Biochenmical Journal.2002, 361: 1-25. [13]王丽慧.N一脱氧核糖转移酶II的克隆表达及其相关应用研究[D].华东理工大学，2011. [14]WEI X, DING Q, Ou L, et al. Two-step enzymatic synthesis of guanine arabinoside[J]. Chinese Journal of Chemical Engineering, 2008, 16(6): 934-937.
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3. 毕业设计（论文）进程安排
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起讫日期 |
设计（论文）各阶段工作内容 |
备 注 |
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2015.12-2016.2 |
文献查阅，完成开题报告，熟悉实验仪器。 |
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2016.3-2016.4 |
核苷磷酸化酶产生菌株筛选以及鉴定 |
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2016.4-2016.5 |
核苷磷酸化酶基因克隆 |
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2016.5-2016.6 |
核苷磷酸化酶表达载体构建 |
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2016.6-2016.6 |
核苷磷酸化酶表达条件优化 |
