论文总字数：18481字

摘 要

本论文的目的是制备功能化石墨烯基纳米材料以及评价其对抗肿瘤的作用。以石墨粉末为原材料，先采取Hummers法制备氧化石墨，经过超声波振荡获得氧化石墨烯（GO）。采用 EDC / Sulfo-NHS催化法制备HA-Ce6，采用溶液共混并水浴超声将HA-Ce6修饰到GO表面，制成功能化石墨烯基纳米材料。利用荧光光谱、紫外光谱（UV）、粒径及电位检测等检测方法对其结构和形态进行表征。GO经HA-Ce6修饰，紫外吸收光谱中两者吸收峰叠加。荧光光谱检测出HA-Ce6修饰到GO后，荧光性减弱，GO对光敏剂Ce6有荧光猝灭作用。利用激光粒度分析仪检测GO和GO-Ce6-HA纳米材料的粒径和电位并进行分析，GO的粒径为160.9nm，GO-Ce6-HA的粒径为255.5nm，HA-Ce6修饰到GO表面后，显著性的降低了GO的电位。HA对肿瘤具有靶向性，Ce6具有光动力效应，GO作为载药分子合成了具备多种功能的新型纳米递药系统，在未来以纳米石墨烯材料为基础的光动力疗法将引起全球范围内的关注，功能化石墨烯基纳米材料将在肿瘤的诊断治疗学中发挥巨大的作用。

关键词：氧化石墨烯（GO） 功能化 光敏剂 抗肿瘤药物

Synthesis and anti tumor of functional graphene based nanomaterials

Abstract

Here, we aim to prepare functionalized graphene based nano materials and evaluate its antitumor efficacy. Graphite powder was used as raw material, the oxidation of graphite was prepared by Hummers method, and then the graphene oxide (GO) was obtained by ultrasonic wave oscillation. The EDC / Sulfo-NHS catalysis method was used for synthesis of HA-Ce6, and it was complexed onto GO by solution blending and ultrasonic bath. The structure and morphology were characterized by fluorescence spectrum, ultraviolet spectroscopy (UV), particle size and zeta potential detection. GO was modified by HA-Ce6, and the absorption peaks in the UV absorption spectrum were added. Fluorescence spectra were detected after HA-Ce6 modification to GO.the fluorescence of HA-Ce6 is decreased and GO has a fluorescence quenching effect of photosensitizer Ce6. Particle size and potential of GO and GO-Ce6-HA nano materials were detected by laser particle size analyzer.The particle size of GO is 160.9nm and The particle size of GO-Ce6-HA is 255.5nm.After the modification of HA-Ce6 to the GO surface, the potential of GO decreased significantly.HA is targeted to cancer, Ce6 has photodynamic effect, and GO is a new type of nano drug delivery system with multiple functions.In the future, nano graphene based photodynamic therapy will attract worldwide attention.Functional graphene based nanomaterials will play an important role in the diagnosis and treatment of tumors.

Key words: Graphene oxide(GO);Functionalization ;photosensitizer;antitumor drug 

目 录

摘 要 I

ABSTRACT II

第一章 文献综述 1

1.1 石墨烯及其衍生物 1

1.1.1 氧化石墨烯的相关性质 1

1.1.2 功能化石墨烯基纳米材料的相关性质 2

1.2 功能化石墨烯基纳米材料研究水平概述 3

1.2.1 功能化石墨烯基纳米材料 3

1.2.2 国内外研究概论 4

1.3 功能化石墨烯基纳米材料在抗肿瘤研究中的应用 5

1.4 光动力疗法概述 6

1.5 本课题的意义 7

1.5.1 本论文的主要内容 7

1.5.2 本论文的意义 7

第二章 实验部分 8

2.1 前言 8

2.2 实验器材 9

2.3 实验试剂 10

2.4 实验过程 11

2.3.1功能化石墨烯基纳米材料的合成 11

2.3.2分析方法 12

第三章 结果与讨论 14

3.1功能化石墨烯及纳米材料的表征 14

3.1.1 紫外可见光谱定性分析 14

3.1.2 荧光光谱定性分析 15

3.1.3 激光粒度分析仪的检测 17

第四章 结论与展望 21

4.1 结论 21

4.2 展望 21

参考文献 22

致谢 24

第一章 文献综述

1.1 石墨烯及其衍生物

石墨烯（Graphene）是从石墨中剥离出来、由碳原子构成的只有一层原子厚度的二维晶体^[1]。石墨烯的二维晶体构造是完美的，其晶格是六个碳原子围成的一个六边形，厚度为一个原子层。碳与碳之间由π键相互连结，结合方法为sp²杂化，这些π键赐予石墨烯极其优异的力学性质与其构造刚性。石墨烯的二维结构和用P轨道电子参与成键电子共轭赋予了其特有的热电及物化性质，故石墨烯具有重要的理论以及现实研究意义。

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