1. 毕业设计（论文）的内容和要求

食品中重金属离子严重危害人类健康。

目前分析食品中重金属离子的方法主要集中在仪器分析方法上，成本高、时间长。

本课题拟建立一种核酸适配体生物传感技术分析食品中as3 的方法。

2. 参考文献

[1] Mccall K A, Huang C, Fierke C A. Function and mechanism of zinc metalloenzymes[J]. Journal of Nutrition, 2000, 130(5S Suppl):1437S. [2] Principles and methods for the assessment of risks from essential trace elements. World Health Organization/IPCS. Geneva World Health Organization . 2002. [3] Malbohan I M, Fialov#225; L. [Trace elements, human nutrition and health][J]. Casop#237;s L#233;kar Ceskych, 1997, 136(11):356-9.. [4] Senesi G S , Baldassarre G , Senesi N , et al. Trace element inputs into soils by anthropogenic activities and implications for human health[J]. Chemosphere, 1999, 39(2):343-77. [5] 白爱梅, 李跃, 范中学.砷对人体健康的危害[J].微量元素与健康研究, 2007, 24 (1) :61-62. [6] Argos M , Kalra T , Rathouz P J , et al. Arsenic exposure from drinking water, and all-cause and chronic-disease mortalities in Bangladesh (HEALS): A prospective cohort study[J]. The Lancet, 2010, 376(9737):252-258. [7] 文卫华. 职业砷接触人群健康危害与遗传毒性研究[D].四川大学,2007. [8] 毛小庆. 水中砷的电化学检测及去除的研究[D].江南大学,2015. [9] 中华人民共和国国家卫生和计划生育委员会.GB 5009.11-2014食品安全国家标准食品中总砷及 无机砷的测定[S].北京:中国标准出版社, 2016. [10] Huang J R, Li P, Wen J H, et al. Determination of arsenic species in mainstream cigarette smoke based on inductively coupled plasma mass spectrometry[J]. Spectroscopy Letters, 2018: 51:6, 252-256. [11] 姜洋,陈维,陆皓昀,杨懂艳,刘兆莹,刘保献,鹿海峰.原子荧光法和电感耦合等离子体质谱法测定地表水中砷、锑对比分析[J].分析仪器,2018(01):105-108. [12] Lee K H, Oshima M, Motomizu S. Inductively coupled plasma mass spectrometric determination of heavy metals in sea-water samples after pre-treatment with a chelating resin disk by an on-line flow injection method[J]. Analyst, 2002, 127(6):769-74. [13] Pereira S D F P, Oliveira G R F, Oliveira J D S, et al. Arsenic spectrophotometric determination in soil of the Santana-AP city using the silver diethyldithiocarbamate (SDDC) modified method.[J]. Acta Amaz, 2009, 39(4):953-960. [14] 吴璇,巫柳彬,边智勤,覃晓东.新银盐法、银盐法测定生活饮用水中砷的效果比较[J].中国卫生检验杂志,2013,23(18):3496-3497. [15] Loska K, Wiechu#322;a D. Comparison of Sample Digestion Procedures for the Determination of Arsenic in Bottom Sediment Using Hydride Generation AAS[J]. Microchimica Acta, 2006, 154(3-4):235-240. [16] 赵云.氢化物-原子荧光法测定地表水中痕量砷和硒[J].水利技术监督,2018(03):44-46. [17] 赵兴康.氢化物原子荧光法测定生活饮用水中的砷[J].云南化工,2018,45(06):136-137. [18] 迟忠云,张志红,候晓芳,刘耀坤.氢化物发生原子吸收法与银盐法测定食品中砷结果比较分析[J].吉林医学院学报(自然科学版),1998(04):57. [19] 张瑛,徐卫东,黄大亮.化妆品中微量砷的微型核反应堆中子活化分析法[J].环境与健康杂志,2003(05):305-306. [20] 赵行文,文立群,卜秋荣.阳极溶出伏安法同时测定水中微量砷、汞的研究[J].广东化工,2017,44(05):51-52. [21] 康燕. 基于核酸适配体的生物传感器的研究[D]. 湖南大学. [22] Kim M , Um H J , Bang S B , et al. Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer[J]. Environmental Science Technology, 2009, 43(24):9335-9340. [23] Sefah K , Phillips J A , Xiong X , et al. Nucleic acid aptamers for biosensors and bio-analytical applications[J]. ANALYST, 2009, 134(9):1765-0. [24] Fu X B , Qu F , Li N B , et al. A label-free thrombin binding aptamer as a probe for highly sensitive and selective detection of lead(ii) ions by a resonance Rayleigh scattering method[J]. The Analyst, 2012, 137(5):1097. [25] Zhai W, Du C, Li X. A series of logic gates based on electrochemical reduction of Pb2 in self-assembled G-quadruplex on the gold electrode.[J]. Chemical Communications, 2014, 50(17):2093-2095.

3. 毕业设计（论文）进程安排

2019年3月：文献综述，确定实验路线； 2019年4月：实验； 2019年5月：论文写作。