1. 毕业设计（论文）的内容和要求

1 文献阅读与归纳，深入了解课题背景 2 毕赤酵母重组菌的构建与诱导表达 3 掌握酶活测定方法 4 独立完成毕业论文的撰写

2. 参考文献

1 Li Y, Zhong K, Hu A, et al. High-level expression and characterization of a thermostable xylanase mutant from Trichoderma reesei in Pichia pastoris[J]. Protein expression and purification, 2015, 108: 90-96. 2 Cayetano-Cruz M, de los Santos A I P, Garc#237;a-Huante Y, et al. High level expression of a recombinant xylanase by Pichia pastoris cultured in a bioreactor with methanol as the sole carbon source: Purification and biochemical characterization of the enzyme[J]. Biochemical engineering journal, 2016, 112: 161-169. 3 Ruanglek V, Sriprang R, Ratanaphan N, et al. Cloning, expression, characterization, and high cell-density production of recombinant endo-1, 4-β-xylanase from Aspergillus niger in Pichia pastoris[J]. Enzyme and Microbial Technology, 2007, 41(1-2): 19-25.

3. 毕业设计（论文）进程安排

2018.12-2019.01 阅读文献，完成开题报告 2019.03-2019.04 重组毕赤酵母构建与验证 2019.05-2019.05 木聚糖酶酶学性质分析 2019.06-2019.06 撰写毕业论文