1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源，查阅关于环糊精葡萄糖苷转移酶的相关文献。

2．文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1]Mckinlay J B, Vieille C, Zeikus J G. Prospects for a bio-based succinate industry[J]. Applied Microbiology Biotechnology, 2007, 76(4):727. [2]Zeikus D H P J G. Utilization of Electrically Reduced Neutral Red by Actinobacillus succinogenes: Physiological Function of Neutral Red, in Membrane-Driven Fumarate Reduction, and Energy Conservation[J]. Journal of Bacteriology, 1999, 181(8):2403. [3]Kim D Y, Yim S C, Lee P C, et al. Batch and continuous fermentation of succinic acid from wood hydrolysate by Mannheimia succiniciproducens, MBEL55E[J]. Enzyme Microbial Technology, 2004, 35(6):648-653. [4]于丽, 姜岷, 马江锋,等. 过量表达Bacillus subtilis磷酸烯醇式丙酮酸羧化激酶对大肠杆菌产琥珀酸的影响[J]. 微生物学通报, 2010, 37(3):0325-0330. [5]Li Q A, Lei J D, Zhang R Y, et al. Efficient decolorization and deproteinization using uniform polymer microspheres in the succinic acid biorefinery from bio-waste cotton (Gossypium hirsutum L.) stalks.[J]. Bioresource Technology, 2013, 135(135):604-609. [6]中国化工万维网[N/OL].http://www.chem.com.cn/(2008-04-25). [7]Anonymous.1,4-Butanediol [J].Chem.Mark Rep, 2005, (268):42. [8]Anonymous.Prices people [J].Chem.Mark Rep, 2005, (267):16-18. [9]Tilton. H .Prices people [J].Chem.Mark Rep, 2003, (264):16-18. [10]Anonymous. Prices people [J].Chem.Mark Rep, 2005, (267):16-18. [11]Dake S B, Gholap R V, Chaudhari R V. Carbonylation of 1,4-butanediol diacetate using rhodium complex catalyst: a kinetic study[J]. Industrial Engineering Chemistry Research, 1987, 26(8):1513-1518. [12]Jiang H, Gong H, Yang Z, et al. Clean Synthesis of adipic acid by direct oxidation of cyclohexene in the absence of phase transfer agents[J]. Reaction Kinetics Catalysis Letters, 2002, 75(2):315-321. [13]Gross R A, Kalra B. Biodegradable Polymers for the Environment[J]. Science, 2002, 297(5582):803-807. [14]Lemme C J, Datta R. A process for the production of succinic acid by anaerobic fermentation[J]. 1992. [15]Kim D Y, Yim S C, Lee P C, et al. Batch and continuous fermentation of succinic acid from wood hydrolysate by Mannheimia succiniciproducens, MBEL55E[J]. Enzyme Microbial Technology, 2004, 35(6):648-653. [16]Okino S, Noburyu R, Suda M, et al. An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum, strain[J]. Appl.microbiol.biotechnol, 2008, 81(3):459-464. [17]Li Q, Siles J A, Thompson I P. Succinic acid production from orange peel and wheat straw by batch fermentations of Fibrobacter succinogenes, S85[J]. Applied Microbiology Biotechnology, 2010, 88(3):671-8. [18]Inui M, Murakami S, Okino S, et al. Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions[J]. Journal of Molecular Microbiology Biotechnology, 2004, 7(4):182-196. [19]Magarifuchi T, Goto K, Iimura Y, et al. Effect of yeast fumarase gene ( FUM1 ) disruption on production of malic, fumaric and succinic acids in sake mash[J]. Journal of Fermentation Bioengineering, 1995, 80(4):355-361. [20]Lin H, Bennett G N, San K Y. Fed‐batch culture of a metabolically engineered Escherichia coli strain designed for high‐level succinate production and yield under aerobic conditions[J]. Biotechnology Bioengineering, 2010, 90(6):775-779.

3. 毕业设计（论文）进程安排

2019.1-2019.2 文献查阅，完成开题报告，熟悉实验仪器； 2019.2-2019.3 完成产琥珀酸放线杆菌的半纤维素（木聚糖）、低聚木糖、木糖利用能力探究。

探究嗜热厌氧杆菌降解条件，并进一步阅读相关文献； 2019.3-2019.4 完成混菌体系的搭建及混菌发酵单因素条件优化； 2019.4-2019.5 完成混菌发酵响应面优化，并完成论文第一章的写作； 2019.5-2019.6 完成论文的写作。