1. 毕业设计（论文）的内容和要求

1 文献阅读与归纳，深入了解课题背景 2 构建过表达多聚磷酸激酶的大肠杆菌重组菌 3 测定相应的酶活 4 独立完成毕业论文的撰写

2. 参考文献

1 Motomura K, Hirota R, Mai O, et al. A New Subfamily of Polyphosphate Kinase 2 (Class III PPK2) Catalyzes both Nucleoside Monophosphate Phosphorylation and Nucleoside Diphosphate Phosphorylation[J]. Applied Environmental Microbiology, 2014, 80(8):2602-2608. 2 Shum K T, Lui E L, Wong S C, et al. Aptamer-mediated inhibition of Mycobacterium tuberculosis polyphosphate kinase 2.[J]. Biochemistry, 2011, 50(15):3261-71. 3 Batten L E, Parnell A E, Wells N J, et al. Biochemical and Structural Characterization of Polyphosphate Kinase 2 from the Intracellular Pathogen Francisella tularensis[J]. Bioscience Reports, 2015, 36(1):e00294. 4 Hao C, Nie K, Li C, et al. Rational design of substrate binding pockets in polyphosphate kinase for use in cost-effective ATP-dependent cascade reactions[J]. Applied Microbiology Biotechnology, 2017, 101(13):5325-5332.

3. 毕业设计（论文）进程安排

2018.01-2018.01 阅读文献，完成开题报告 2018.02-2018.03 重组菌的构建与验证 2018.04-2018.04 酶活检测 2018.05-2018.06 撰写毕业论文