1. 毕业设计（论文）的内容和要求

一、论文内容： 随着代谢工程的迅猛发展及生物合成学的崛起，人类改造微生物作为细胞工厂进行生物制造的能力显著提高。

当前生物制造的原料仍以粮食为主，随着粮食危机日益家具以及其价格不断攀升，以粮食为原料的生物制造正面临严峻考验。

因此，为了解决原料来源问题，实现生物制造可持续发展，目前逐渐将”甲醇经济”发展为合理的可替代能源#8212;#8212;以甲醇为原料，缓解化石能源的紧缺。

2. 参考文献

［1］ Wu T Y, Chen C T, Liu T J, et al. Characterization and evolution of an activator-independent methanol dehydrogenase from Cupriavidus necator, N-1[J]. Applied Microbiology and Biotechnology, 2016, 100(11):1-15. ［2］ M#252;ller J E, Meyer F, Litsanov B, et al. Engineering Escherichia coli for methanol conversion[J]. Metabolic Engineering, 2015, 28:190#8211;201. ［3］ Whitaker W B, Jones J A, Bennett R K, et al. Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli[J]. Metabolic Engineering, 2016. ［4］Ochsner A M, Sonntag F, Buchhaupt M, et al. Methylobacterium extorquens: methylotrophy and biotechnological applications.[J]. Applied Microbiology and Biotechnology, 2015, 99(2):517-534. ［5］Yurimoto H, Kato N, Sakai Y. Genomic organization and biochemistry of the ribulose monophosphate pathway and its application in biotechnology.[J]. Applied Microbiology and Biotechnology, 2009, 84(3):407-416. ［6］ 张雪, 温廷益. Red重组系统用于大肠杆菌基因修饰研究进展[J]. 中国生物工程杂志, 2008, 28(12):89-93. ［7］ Song Y, Hoggard J C, Lidstrom M E, et al. Comprehensive discovery of 13 C labeled metabolites in the bacterium Methylobacterium extorquens, AM1 using gas chromatography#8211;mass spectrometry[J]. Journal of Chromatography A, 2013, 1317:175-185. ［8］Price J V, Chen L, Whitaker W B, et al. Scaffoldless engineered enzyme assembly for enhanced methanol utilization[J]. 2016, 113(45). ［9］Arfman N, Hektor H J, Bystrykh L V, et al. Properties of an NAD(H)-containing methanol dehydrogenase and its activator protein from Bacillus methanolicus.[J]. European Journal of Biochemistry, 1997, 244(2):426#8211;433. ［10］ 张应年, 文婕英, 朱彦荣,等. 氧化品红亚硫酸比色法测定化妆品中甲醇含量[J]. 广东化工, 2008, 35(10):121-123. ［11］Yurimoto H, Kato N, Sakai Y. Assimilation, dissimilation, and detoxification of formaldehyde, a central metabolic intermediate of methylotrophic metabolism[J]. The Chemical Record, 2005, 5(6):367-75. ［12］Schrader J, Schilling M, Holtmann D, et al. Methanol-based industrial biotechnology: current status and future perspectives of methylotrophic bacteria.[J]. Trends in Biotechnology, 2009, 27(2):107-15. ［13］张琪, 胡又佳, 陈习平,等. 基于易错PCR的菌丝霉素的定向进化[J]. 中国医药工业杂志, 2014, 45(6). ［14］Gao X, Yang S, Zhao C, et al. Artificial multienzyme supramolecular device: highly ordered self-assembly of oligomeric enzymes in vitro and in vivo.[J]. Angewandte Chemie, 2014, 53(51):14027#8211;14030.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 2016.12.20-12.27 选题，与导师讨论题目 2017.12.30 导师下达任务书 2016.12.30-2017.1.9 根据任务书要求，查找相关文献资料，完成任务书 2017.01.10-2017.01.13 提交开题报告 2017.01.14-05.23 完成试验并提交论文第一稿 2017.05.24-05.27 根据导师意见修改论文第一稿，完成并提交第二稿 2017.05.28-06.1 根据导师意见修改论文第二稿，完成并提交第三稿 2017.06.1-06.7 完成论文的审阅，准备答辩