摘 要

本文利用平板划线法和稀释涂布平板法从污水厂筛选耐盐高效有机物降解好氧菌。实验表明，在18h时，耐盐好氧菌的COD去除率高达80%左右，还有在200g NaCl的LB固体培养基上仍大约有5个单菌落，这说明细菌在较高盐度下表现出对有机物较好的降解能力。在此基础上，针对某饮料瓶清洗废水，设计了接触氧化工艺处理，进水BOD₅为1000 mg/L，出水BOD₅为400 mg/L，BOD₅去除率为60%，这说明该工艺能够有效用于该废水的处理。

关键词：好氧菌；高盐废水；接触氧化

Culture of salt-tolerant high efficient organic degrading aerobic bacteria and contact oxidation process design

Abstract

In this paper, salt-tolerant and high-efficiency organic degrading aerobic bacteria were screened from wastewater treatment plant by plate marking method and dilution coating plate method. The results showed that the removal rate of COD of halophilic aerobic bacteria was about 80% at 18 h, and there were still about 5 single colonies on the LB solid medium of 200g NaCl, which indicated that the bacteria showed a better ability to degrade organic matter at higher salinity. On this basis, the contact oxidation process was designed to treat a bottle washing wastewater. The BOD5 of influent was 1000 mg / L, and the removal rate of effluent BOD5 was 400 mg / L, the removal rate of BOD5 was 60%. It is shown that the process can be effectively used in the treatment of the wastewater.

Keywords: aerobic formers; high salinity wastewater; contact oxidation

目录

摘要.................................................................................................................................I

Abstract........................................................................................................................II

第一章 综述..................................................................................................................1

1.1引言.........................................................................................................1

1.2接触氧化工艺研究现状.........................................................................1

1.2.1混凝-生物接触氧化-臭氧氧化法...............................................1

1.2.2砾间接触氧化法...........................................................................2

1.2.3两级接触氧化法...........................................................................3

1.2.4预处理—生物接触氧化反应器法...............................................4

1.2.5生物漂带接触氧化工艺...............................................................5

1.2.6水解酸化—生物接触氧化工艺...................................................5

1.3耐盐高效有机物降解好氧菌的培养.....................................................6

1.4本文目的和研究内容.............................................................................7

第二章 实验部分..........................................................................................................8

2.1实验仪器与试剂.....................................................................................8

2.2实验内容.................................................................................................8

2.2.1废水取样.......................................................................................8

2.2.2实验方法.......................................................................................8

2.2.3耐盐好氧菌有机物去除能力的测定...........................................9

2.2.4盐度对耐盐好氧菌的影响...........................................................9

2.2.5 COD的测定....................................................................................9

2.2.6 COD去除率的计算......................................................................10

第三章 实验结果与讨论............................................................................................12

3.1耐盐好氧菌有机物去除能力的测定...................................................12

3.2盐度对耐盐好氧菌的影响...................................................................15

第四章 接触氧化工艺的设计....................................................................................17

4.1已知数据...............................................................................................17

4.2结构设计...............................................................................................17

4.2.1容积负荷.....................................................................................17

4.2.2平面尺寸计算.............................................................................17

4.2.3需气量.........................................................................................18

4.2.4平面布置.....................................................................................18

4.2.5进出水系统.................................................................................19

4.2.6曝气系统设计.............................................................................20

4.2.7填料设计.....................................................................................20

4.2.8草图.............................................................................................20

第五章 结论与展望....................................................................................................21

参考文献......................................................................................................................22

致谢..............................................................................................................................24

第一章 综述

1.1引言

接触氧化工艺是一种于20世纪70年代初开创的污水处理技术，该工艺采用固定式生物填料作为微生物的载体，生长有微生物的载体淹没在水中，曝气系统为反应器中的微生物供氧。

我国在很早之前就开始熟练运用此技术，我国科技工作者在全国范围内地下水除铁实现了从自然氧化法向接触氧化法的转变, 曝气后含锰地下水在接触氧化除锰活性滤膜的铁细菌生物催化和化学催化的共同作用下 ,实现了氧化除锰的目的。